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The ZetaView® is installed in the best laboratories in the world and is enjoying growing popularity. Through collaborations with research institutes, PMX is increasingly working on new applications. The standardization of processes and analyzes will progress rapidly over the next years. Robust design and partial automation contribute to the safe and fast analysis. Demand for GMP, IQ/OQ/PQ and CFR 21 Part 11 continues to increase.

ZetaView® BASIC NTA - Nanoparticle Tracking Video Microscope PMX-120

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

Nanoparticle Tracking Analysis (NTA) captures the Brownian motion of each particle in the video. Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined. Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

All these analyzes are carried out quickly and statistically reliably as required in the scattered light or fluorescence mode.

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labeled with corresponding intercalating dyes.

Depending on the type of sample and the measuring mode, the measuring range is between 15 nm and 5 μm.

The model PMX-120 BASIC is the platform, on which the other two are built on: PMX-220 TWIN and PMX-420 QUATT. Therefore, the series offers upgradeability making budgetary decisions easier. The benefits mentioned below apply for all three models

ZetaView® TWIN – NTA Nanoparticle Tracking - Video Microscope PMX-220

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are in the growing interest of the biosciences and nanomedicine. With the NTA technology, particles are individually measurable in a physiological buffer and you observe what you measure in a video. A new step forward in convenience and speed for fluorescence studies of EV-antibody conjugates has been achieved with the ZetaView® TWIN. Lasers and fluorescence filters are pre-selectable via the software. This procedure saves time and sample.

Typical application

Determining the absolute concentration is one of the strengths of the NTA technique. In scatter mode, the concentration of all particles is measured. Changing subsequently to the fluorescence mode, marked biologic nanoparticles are tracked. By using two lasers within a SOP assisted sequence, it is possible to differentiate between two different fluorescence dyes in the same sample. Phenotyping of tetraspanins for instance, on vesicles and virus is facilitated, positive and isotype control as well. To prepare instrument settings, standard EVs may be used (HBM HansaBioMed https://www.hansabiomed.eu/).

ZetaView® QUATT– NTA Nanoparticle Tracking - Video Microscope PMX-420

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are in the growing interest of the biosciences and nanomedicine. With the NTA technology, particles are individually measurable in a physiological buffer and you observe what you measure in a video. A new step forward in convenience and speed for fluorescence studies of EV-antibody conjugates has been achieved with the ZetaView® QUATT Lasers and fluorescence filters are pre-selectable via the software. This procedure saves time and sample.

Typical application

Determining the absolute concentration is one of the strengths of the NTA technique. In scatter mode, the concentration of all particles is measured. Changing subsequently to the fluorescence mode, marked biologic nanoparticles are tracked. By using four lasers within a SOP assisted sequence, it is possible to differentiate between four different fluorescence dyes in the same sample. Phenotyping of tetraspanins for instance, on vesicles and virus is facilitated, positive and isotype control as well. To prepare instrument settings, standard EVs may be used (HBM HansaBioMed https://www.hansabiomed.eu/).

Technical Information and Applications